Microscopy Core Facility (MFC)

The multi-user Microscopy Core Facility (MCF) provides a broad spectrum of equipment for advanced microscopy studies. The MCF is equipped with devices for in vivo experiments (two photon), a confocal setup for multicolor-imaging of fixated preparations and a general fluorescence microscope.
 
For more information please visit the MCF webpage.
 
 
 
Systems accessible to all researchers

The following systems are registered in the DFG Database for Research Infrastructure. Costs for service are funded by the DFG. Before you apply for funds please contact the facility for details.

Confocal microscopy system SPE [Leica]
Optimized for fixated preparations
Excitation
Epifluorescence uv (340-380nm)
Epifluorescence blue (450-490nm)
Epifluorescence green (515-560nm)
Single-photon
Solid-state Laser (405, 488, 532, 635nm)
Detection
1 PMT 430 - 750nm spectral descanned
Equipment
Upright microscope (Leica DM 2500)
10x dry, 20x, 40x, 63x oil immersion objectives
Condensor dry NA 0.9
z-galvo table, mechanical xy-table
 
Neurolucida system [Olympus]
Optimized for fixated preparations
Excitation
Epifluorescence uv (360-370nm)
Epifluorescence blue (470-495nnm)
Epifluorescence green (540-550nm)
Detection
CCD Camera color
Emission blue (420-460nm)
Emission green (510-550nm)
Emission red (600-660nm)
Equipment
Upright microscope (Olympus BX61)
2.5x, 10x, 20x, 40x, dry 60x, 100x,
oil immersion objectives
 
Fluorescence system [Olympus]
Optimized for fixated preparations
Excitation
Epifluorescence uv (360-370nm)
Epifluorescence blue (470-495nnm)
Epifluorescence green (540-550nm)
Detection
CCD Camera monochrome or color
Emission blue (420-460nm)
Emission green (510-550nm)
Emission red (575-625nm)
Equipment
Upright microscope (Olympus BX 61)
2.5x, 10x, 20x, 40x, dry 60x, 100x, oil immersion objectives
 
 
The following system is not yet registered with the DFG and free of charge until further notice.
 
Microdissection system [Arcturus]
Laser
UV cutting laser   solid state 355 nm
Capture laser   laser diode 810 nm
Filter Fluorescence
Color Excitation Emission
red 570-630 nm > 655 nm
green 503-548 nm > 565 nm
blue 455-495 nm > 510 nm
uv 340-390 nm > 410 nm
Bright Field
Phase contrast and DIC
100W Halogen lamp
Objectives
Upright microscope Nikon
4x; 20x; 60x; 100x dry
 
Systems preferentially accessible to researchers associated with NeuroCure

The following systems are registered in the DFG Database for Research Infrastructure. Costs for service are funded by the DFG. Before you apply for funds please contact the facility for details.

Confocal microscopy system SP5 [Leica]
Optimized for fixed preparation
Exitation
Epifluorescence uv (340-380 nm)
Epifluorescence blue (450-490 nm)
Epifluorescence green (515-560 nm)
Laser diode (405)
Single-photon laser Ar (458, 476, 488, 496, 514 nm)
Solid state laser (568 nm), HeNe laser (633 nm)
Detection
3 PMT 400-800nm spectral descanned
2 APD for green and red emission
Equipment
Upright microscope (Leica DM 6000)
10x dry, 20x imm, 63x oil objectives
mechanical xy-table
 
 
Confocal microscopy system SP8 [Leica]
Optimized for fixed preparation
Exitation
Epifluorescence uv (340-380 nm)
Epifluorescence blue (450-490 nm)
Epifluorescence green (515-560 nm)
Solid sate laser (405, 488, 552, 635 nm)
Detection
3 PMT 400-800nm spectral descanned
2 HYD (high sensitive) 400-800nm spectral descanned
Equipment
inverse microscope (Leica DMI 6000)
20x imm, 63x oil objectives
z galvo table, mechanical xy-table
 
 
 
The following systems are not yet registered with the DFG and free of charge until further notice.
 
2-Photon in vivo system [Femtonics]
Optimized for in vivo preparations
Excitation
Two photon IR (690-1040nm)
Detection
Non-descanned-PMT green (480-580nm)
Non-descanned-PMT red (590-710nm)
Equipment
Upright microscope (Olympus BX 61)
10x and 40x water immersion objectives
L&N table and micromanipulators
 
2-Photon in vitro system [Femtonics]
Optimized for in vitro preparations
Excitation
Two photon IR (710-950nm) and
Two photon IR (720-950nm)
Detection
Non-descanned-PMT green (480-580nm)
Non-descanned-PMT red (590-710nm)
Equipment
Upright microscope (Olympus BX 61)
10x and 40x water immersion objectives
L&N table and micromanipulators
 
Gated STED Set-Up [Leica]
High resolution Microscope optimized for fixated preparations
Excitation
Epifluorescence blue (450-490nm)
Epifluorescence green (515-560nm)
Single photon Ar (458, 476, 488, 496, 514nm)
Solid state (568nm), NeNe laser (633nm)
CW gated STED laser (592nm)
White light laser (470-670nm)
Detection
2 PMT 400-800nm spectral descanned
2 HYD (high sensitive) 400-800nm spectral descanned
Equipment
inverse microscope (Leica DMI 6000)
10x dry, 20x imm, 63x oil Immersion objectives
z galvo table, mechanical xy-table
 
General information
Laser safety

The Tsunami (of Spectra-Physics) and Chameleon Ultra (of Coherent) lasers are 4 class high power lasers, whose beams can cause badly eye or skin damage.
In our facility the laser beam is completely enclosed and shielded up to the microscope objectives.


To prevent exposure to both direct and reflected beams please:

  • don’t enter the room, if there are warning notices at the door
  • use only what you are introduced in
  • consider the effects before each action
  • don’t take any covers from the equipment
  • don’t install or uninstall optical elements (objectives excepted)
  • don’t put mirrors or other reflective things under the microscope objectives
Software
Software to download
Please select the free software
 
Resolution
Notes on confocal resolution

Lateral resolution:

FWHM = 0.4 * λ / NA

Axial resolution:

FWHM = 0.45 * λ / n (1-cos α)

NA = n*sinα

FWHM:
full width half maximum (or spatial resolution)

NA:
numeral aperture of the chosen objective

n:
refraction index of the sample medium (for air: n = 1, for immersion oil: n = 1.5)

λ:
laser wavelength

The improvement in spatial resolution corresponds lateral to 1.4x and axial to 6x compaired with the conventional fluorescence microscopy. You can adjust the spatial resolution in the LCS software using “Zoom” and “Format”.
As a simple rule you can use:

Lateral: resolution/3 = optimal size of the voxel
Axial: resolution/3 = optimal choice of the z-scan

Please note: the spatial resolution depends on the used wavelength.